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The standard curve was generated by plotting the fluorescence intensity against the <t>dsDNA</t> concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean <t>PicoGreen</t> fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
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The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

Journal: Bio-protocol

Article Title: A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters

doi: 10.21769/BioProtoc.5680

Figure Lengend Snippet: The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

Article Snippet: Picogreen dsDNA quantitation reagent (YEASEN, catalog number: 12641ES04) Western blotting 17.

Techniques: Generated, Fluorescence, Concentration Assay